| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Clastogenic activity of 2-chlorodeoxyadenosine in mammalian somatic cells
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| Autores: |
Antonucci,Gilmara Ausech
Takahashi,Catarina Satie
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| Data: |
2005-01-01
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| Ano: |
2005
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| Palavras-chave: |
Human lymphocytes
2-CdA
Chromosomal aberrations
SCE
Cell cycle
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| Resumo: |
The base analogue 2-chlorodeoxyadenosine (2-CdA) used for therapy in chronic resistant and advanced lymphoproliferative disorders, is cytotoxic for both dividing and non-dividing lymphocytes. The present work evaluated the clastogenic potential of this drug in vitro in human lymphocytes in culture and in vivo in BALB/c mice bone marrow cells. In human lymphocytes, the clastogenic effect of 2-CdA was studied in G1, S and G2 phases of the cell cycle, using three different concentrations (10, 20 and 40 mug/mL). The endpoints analyzed included mitotic index (MI), proliferation index (PI), sister chromatid exchange (SCE), and chromosomal aberration (CA). Statistical analysis by a variance (ANOVA) test showed a significant increase (p < 0.05) in CA frequencies for cells treated during the S phase, but the MI did not vary. The concentrations tested did not produce a significant increase in the mean frequency of SCEs, nor did they change the cell PI in the G1 and S phases. The concentrations in vivo tested were 0.25, 0.375 and 0.5 mg/kg body weight. In this assay, alterations in CA frequencies and MI were not observed at the dose levels tested. Therefore, the results indicate a clastogenic effect of 2-CdA in human lymphocyte cultures.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200027
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572005000200027
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.28 n.2 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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